ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI FRAKSI n-BUTANOL DAUN TIN (Ficus carica L.) VARIETAS BROWN TURKEY
DOI:
https://doi.org/10.51887/jpfi.v8i1.625Keywords:
Keywords: Flavonoids, Antioxidants, IC50, Ficus carica L.Abstract
Tin leaf (Ficus carica L.) is one of the traditional medicines that has potential as an antioxidant. The purpose of this study was to isolate secondary metabolites and to test the antioxidant activity of pure isolates from the n-butanol fraction of tin leaves. Extraction was carried out by the fractionation method using n-hexane, ethyl acetate and n-butanol. Then the n-butanol fraction was separated by column chromatography and antioxidant activity was tested using the DPPH method. Pure isolate compounds labeled HNF-3, in the form of crystals, white with a melting point 189-191 ° C. Based on phytochemical examination data analysis, UV spectroscopy, FT-IR and 1H-NMR it can be concluded that the HNF-3 compound is a flavonoid glycoside group and has a moderate category of antioxidant activity with an IC50 value of 160.613 µg / mL.
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References
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Blunder, M., Orthaber, A., Rudolf, B., Bucar, F. 2017. Efficient Identification of Flavones, Flavanones and Their Glycosides in Routine Analysis via Off-line Combination of Sensitive NMR and HPLC Experiment. Food Chemistry 218, 600-609.
Chatuverdula, V.S.P., Miguel, R.I.S., Prakash, I. 2012. Chemical Constituents From Polar Fraction of Rubus Suavissimus. Organic Chem Current Res. 1:1
Coates, J. 2006. Interpretation of Infrared Spectra, A Practical Approach. Encyclopedia of Analytical Chemistry, 10815–10837.
Joseph, B., and Raj, S. J. 2011. Pharmacognostic and Phytochemical Properties of Ficus carica Linn–An Overview. International Journal of Pharmatech Research. 3 (1): 8-12.
Kedare, S. B., dan Singh, R. P. 2011. Genesis and Development of DPPH Method Of Antioxidant Assay. J Food Sci Technol, 48(4), 412–422.
Khatimah, D. K. 2018. Uji Aktivitas Antioksidan Ekstrak Dan Fraksi Daun Tin (Ficus carica L.) Varietas Brown Turkey Dengan Metode Dpph (1,1- Diphenyl-2-Picrylhydrazyl). Skripsi, Pekanbaru, STIFAR
Mahmoudi, S., Khali, M., Benkhaled, A., Benamirouche, K., Baiti, I. 2016. Phenolic and Flavonoid Contents, Antioxidant and Antimicrobial Activities of Leaf Extracts from Ten Algerian Ficus carica L. Varieties. Asian Pacific Journal of Tropical Biomedicine. 6 (3): 239-245.
Molyneux, P. 2004. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Journal Songklanakarin Science Technology, 26 : 212-219.
Muhtadi, E. H., Syah, Y. M., Juliawaty, L. D., Din, L. B., Latip, J., 2007, Senyawa oligomer resveratrol dari kulit kayu Dipterocarpus retusus Blume dan efek sitotoksiknya terhadap sel murin leukemia P388, J Pharmacon, Vol. 8 (1), Hal. 6-12.
Mustarichie, R., Runadi, D., Ramdhani, D. 2017. The Antioxidant Activity and Phytochemical Screening of Ethanol Extract, Fractions of Water, Ethyl Acetate, and n-Hexane From Mistelotoe Tea (Scurrula atropurpurea BL.Dans). Asian Journal of Pharmaceutical and Clinical Research, 10(2): 343-347.
Nicotra, G., Vicentini, S., Mazzolari, A. 2010. Ficus carica. Nutra Foods. 9 (3) :27-30.
Oliveira, A, P., Valent˜ao, P., Pereira, J. A., Silva, B. M., Tavares, F., and. Andrade, P. B, 2009. “Ficus carica L.: metabolic and biological screening,” Food and Chemical Toxicology, vol. 47, no. 11, pp. 2841–2846.
Packer, Lester. 2001. Flavonoids and Other Polyphenols Vol. 335. Academic Press. New York.
Redha, A. 2010. Flavonoid : Sturktur, Sifat Antioksidatif dan Peranannya Dalam Sistem Biologis. Jurnal Belian, 9(2), 196-202.
Rustaman, Abdurahman, M., dan Jamaludin, A., 2006. Skrinning Fitokimia Tumbuhan di Kawasan Gunung Kuda Kabupaten Bandung sebagai Penelaahan Keanekaragaman Hayati, Lembaga Penelitian Universitas Pandjajaran, Bandung.
Salamah, N., Widyaningsih, W., Izati, I., Susanti, H., 2015. Aktivitas Penangkapan Radikal Bebas Ekstrak Etanol Ganggang Hijau Spirogyra sp. dan Ulva lactuca dengan Metode DPPH, Jurnal Ilmu Kefarmasian Indonesia. 13 (2) : 145-150.
Sayuti, K. dan Yanrina, R. 2015. Antioksidan Alami dan Sintetik. Andalas University Press, Padang.
Sirisha, N., Sreenivasulu, M., Sangeeta, K., Madhusudhana, C. 2010. Antioxidant Properties of Ficus Species – A Review. International Journal of PharmTech Research. 2 (4): 2174-2182.
Stover, Ed and Aradhya, M. 2007. The Fig: Overview of an Ancient Fruit. Hort Science. 42 (5).
Suhartati, T. 2017. Dasar-dasar Spektrofotometri UV-Vis dan Spektrofotometri Massa. Anugrah Utama Raharja. Lampung.
Tian, J., Zhang Y., Yang, X., Ruii, K., Tang, X., Ma, J., Chen, J., Xu, H., Lu, L., Wang, S. 2014. Ficus carica Polysaccharides Promote The Maturation and Function of Dendritic Cells. International Journal Medical Science. (15): 12469-12479.
Tjitrosoepomo, G. 1994. Taksonomi Tumbuhan Obat. Gajah Mada University Press. Yogyakarta.
Vihakas, M. 2014. Flavonoids And Other Phenolic Compounds: Characterization And Interactions With Lepidopteran And Sawfly Larvae. Department of Chemistry, University Of Turku.
Zhang Q., Junzeng Z., Jingkai S, Anggelica S., Dorothy A., 2006. A simple 96- Well Microplate Method For Estimation of Total Polyphenol Content In Seawds. Journal of Applied Phycology. (18): 445-450.
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Published
01-09-2019
How to Cite
Prayoga, H. N., & rahmawati, noveri. (2019). ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI FRAKSI n-BUTANOL DAUN TIN (Ficus carica L.) VARIETAS BROWN TURKEY. Jurnal Penelitian Farmasi Indonesia, 8(1), 24–31. https://doi.org/10.51887/jpfi.v8i1.625
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