ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN FRAKSI ALKALOID DARI EKSTRAK METANOL KULIT BATANG BUNGA KUPU-KUPU (Bauhinia semibifida Roxb.)
Keywords:
Alkaloids, Antioxidant, Bauhinia semibifida Roxb., IC50.Abstract
Bauhinia semibifida Roxb. is a plant has potensial as antioxidant. The purpose of this research is to isolate alkaloids fraction from methanol extract and to examine the activity of antioxidant with DPPH method. The extraction was done by using maceration method with metanol solvent and an acid-base was performed on the extract with acid citric 2% and amonia, and fractionation with ethyl acetat and butanol solvents. The ethyl acetat fraction was separated by column chromtography. The isolates from column chromatography was labeled SA fraction, which a yellow colour oil. Based on phytochemicals data analysis, the spectroscopy of UV-Vis, FT-IR, and LC-MS, it can be concluded that the SA fraction is a compound has a NH functional group (alkaoids), and has very weak antioxidant activity with IC50 429,64 mg/mL.
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References
Fadhli, H., Nurdin, A.N. & Octaviani, M. 2019. Potensi Antioksidan dari Ekstrak Kulit Batang Bauhinia semibifida Roxb. Jurnal Ilmiah Ibnu Sina, 4(1): 77–87.
Fadhli, H., Soeharto, A.B.R. & Windarti, T. 2018. Uji Aktivitas Antioksidan Kulit Buah Pulasan (Nephelium mutabile Blume) dan Bunga Turi Putih (Sesbania grandiflora) dengan Metoda DPPH. Jurnal Katalisator, 3(2): 114–124.
Fitmawati 2017. Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago. Biosaintifika, 9(2): 325–331.
Juma, B.F. & Majinda, R.R.T. 2004. Erythrinaline Alkaloids From The Flowers and Pods of Erythrina lysistemon and Their DPPH Radical Scavenging Properties. Phytochemistry, 65(10): 1397–1404.
Kedare, S.B. & Singh, R.P. 2011. Genesis and Development of DPPH Method of Antioxidant Assay. Journal of food science and technology, 48(4): 412–422.
Krishnaveni, M. 2015. Phytochemical study of Bauhinia purpurea Linn. Stem. Research Journal of Pharmacy and Technology, 8(11): 1555–1559.
Sayuti, K. & Yenrina, R. 2015. Antioksidan alami dan sintetik. Padang. Universitas Adalas.
