IDENTIFIKASI DAN UJI RESISTENSI BAKTERI PADA PASIEN ULKUS DIABETIKUM DI BANGSAL INTERNE RSUP DR. M. DJAMIL PADANG
DOI:
https://doi.org/10.51887/jpfi.v8i2.550Abstract
Diabetic ulcers is one of diabetic mellitus’s chronic complication such as open wound on the skin and can be accompanied by necrotic tissues. High blood glucose levels become a strategic area to the development of bacteria. This study aims to determine the bacterial agent that cause of diabetic ulcer and its resistance to antibiotics. The design of this study was true experiment with diabetic ulcers patients as the subject in the ward interne at RSUP Dr. M. Djamil Padang on period February - April 2019. In this study 10 samples of diabetic ulcer patients were obtained, then bacterial identification and antibiotic resistance test was done against 5 types of antibiotics ciprofloxacin, cefotaxime, meropenem, gentamicin and vankomicin. Consecutively most of bacteria found were Klebsiella pneumonia (40%), Staphylococcus epidermidis (60%). Proteus vulgaris (10%), Proteus mirabilis (10%), Pseudomonas aeruginosa (10%) and Staphylococcus aureus (10%). Ciprofloxacin and Cefotaxime had the highest level of bacterial resistance (40%), while Vancomicin is effective to used in Staphylococcus aureus and Staphylococcus epidermidis.
Downloads
Download data is not yet available.
References
Catheter-Associated Urinary Tract Infection In The Intensive Care Unit Of Assiut University Hospital. Al Azhar Assiut Medical Journal. 14(2):52-58.
Bonang., Gerard., Koeswardono., dan Enggar, S. 2002. Mikrobiologi Kedokteran untuk Laboratorium dan Klinik. Jakarta : Gramedia.
Clinical and Laboratory Standards Institute (CLSI). 2014. Performance Standards for Antimicrobial Susceptibility Testing : Twenty Fourth International Supplement, Clinical and Laboratory Standards Insitute, Wayne.
Dirk, S. Y. P. R., dan Waworuntu, L. V. 2014. Sensitivitas Antibiotik terhadap Bakteri yang Diisolasi dari Ulkus Diabetika di RSUD Abepura, Jurnal Biologi Papua : Kota Jayapura, 6(2): 53–59.
Ferreira, C. M., Ferreira, W. A., Almeida, N. C. O. S., Naveca, F. G., Barbosa, M. G. V. 2011. Extended-Spectrum Beta-Lactamase-Producing Bacteria Isolated From Hematologic Patients In Manaus, State Of Amazonas, Brazil. Brazilian Journal Of Microbiology. 42(3):1076–1084.
Gaol, Y. E. L., Erly., dan Elmatris, S.Y. 2014. Pola Resistensi Bakteri Aerob Pada Ulkus Diabetik Terhadap Beberapa Antibiotika di Laboratorium Mikrobiologi RSUP Dr. M. Djamil Padang Tahun 2011-2013, Jurnal Kesehatan Andalas, 6(1).
Gilman, Goodman A. 2012, Goodman & Gilman. Dasar Farmakologi Terapi, Ed 10, Jakarta :EGC
Harley, P. 2002 Laboratory Exercises in Microbiology, 5th edition, 257-260. New York: McGraw-Hill Companies.
Hastuti, R., T. 2008. Faktor-Faktor Risiko Ulkus Diabetika pada Penderita Diabetes Mellitus (Studi Kasus di RSUD Dr. Moewardi Surakarta). [Tesis]. Universitas Diponegoro, Semarang.
International Diabetes Federation (IDF). 2011. IDF Diabetes Atlas, 5th Edition, International Diabetes Federation.
Jawetz., Melnick., dan Adelberg. 2010. Mikrobiologi Kedokteran. Jakarta: EGC.
Kemenkes RI. 2013. Riset Kesehatan Dasar. Badan Penelitian & Pengembangan Kementrian Kesehatan RI, Jakarta.
Kontopoulou, K., Protonotariou, E., Vasilakos, K., Kriti, M., Koteli, A., Antoniadou, E., Safianou, D. 2010. Hospital Outbreak Caused By Klebsiella pneumonia Producing KPC-2 Beta-lactamase Resistant To Colistin. J Hosp Infect, 76(1) : 70-73
Lipsky, B., A., Berendt A., R., Cornia P., B., Pile, J., C., Peters, E., J., G., dan Armstrong, D., G. 2012. Infection Diseases Society of America Clinical Practice Guideline for the Diagnosis and Treatment of Diabetic Foot Infections, Clinical Infectious Diseases. 54 (12) : 132-173.
Ludden, C., Cormican, M., Vellinga, A., Johnson, J. R., Austin, B., dan Morris, D. 2015. Colonisation With ESBL – Producing And Carbapenemase –Producing Enterobacteriaceae, Vancomycin – Resistant Enterococci, And Meticillin – Resistant Staphylococcus Aureus In A Long-Tern Care Facility Over One Year. BMC Infect Dis. 15(168).
McPhee, S. J., dan Ganong, W. F. 2010. Patofisiologi Penyakit Pengantar Menuju Kedokteran Klinis, Edisi 5. Jakarta: EGC.
Nur, A., dan Marissa, N. 2016. Gambaran Bakteri Ulkus Diabetikum di Rumah Sakit Zainal Abidin dan Merauxa Tahun 2015, Buletin Penelitian Kesehatan. 44(3) : 187-196.
Patil, Y., Khadse, R., Chavan, S., dan Raut,S. 2018, Bacteriological Profil of Diabetic Foot Infections, European Journal of Pharmaceutical and Medical Research. 5 (6) : 631-635.
Riset Kesehatan Dasar (Riskesdas). 2013. Pedoman Pewancara Petugas Pengumpul Data, Badan Litbangkes Depkes RI, Jakarta.
Rizal. 2010. Microbial Pattern And Antimicrobial Resistance Of Isolates Collected From Various Specimen In DR. Oen Solo Baru Hospital, Sukoharjo, The Indonesian Journal Of Medical Science. 1(7) : 392.
Setianingsih, Y., Fadraersada, J., Ibrahim, A., dan Ramadhan, A. M. 2016. Pola Resistensi Bakteri Terhadap Antibiotik Pada Pasien Diabetic Foot Di RSUP Abdul Wahab Sjahranie Samarinda Periode Agustus-Oktober 2016, Prosiding Seminar Nasional Kefarmasian ke-4: 407-414.
Setiawan, D. S. 2010. Faktor Resiko Koloni sasi Enterobacteriaceae Pada Nasofaring Manusia Dewasa, Universitas Diponegoro, Semarang.
Suhartati, R., dan Faidah, E. N. 2014. Identifikasi Bakteri Oxacillin Resistant Staphylococcus Aureus (Orsa) Pada Ulkus Penderita Diabetes Mellitus Di Ruang Perawatan Bedah Rumah Sakit Umum Daerah (RSUD) Tasikmalaya, Jurnal Kesehatan Bakti Tunas Husada. 11 (1) : 161-167.
Waspadji, S. 2012. Komplikasi Kronik Diabetes: Mekanisme Terjadinya, Diagnosis & Strategi Pengelolaan. Jakarta: EGC.
Yunir, Soebardi. 2006. Buku Ajar Ilmu Penyakit Dalam Jilid III Ed. 4. Jakarta: Balai penerbit FKU.
Bonang., Gerard., Koeswardono., dan Enggar, S. 2002. Mikrobiologi Kedokteran untuk Laboratorium dan Klinik. Jakarta : Gramedia.
Clinical and Laboratory Standards Institute (CLSI). 2014. Performance Standards for Antimicrobial Susceptibility Testing : Twenty Fourth International Supplement, Clinical and Laboratory Standards Insitute, Wayne.
Dirk, S. Y. P. R., dan Waworuntu, L. V. 2014. Sensitivitas Antibiotik terhadap Bakteri yang Diisolasi dari Ulkus Diabetika di RSUD Abepura, Jurnal Biologi Papua : Kota Jayapura, 6(2): 53–59.
Ferreira, C. M., Ferreira, W. A., Almeida, N. C. O. S., Naveca, F. G., Barbosa, M. G. V. 2011. Extended-Spectrum Beta-Lactamase-Producing Bacteria Isolated From Hematologic Patients In Manaus, State Of Amazonas, Brazil. Brazilian Journal Of Microbiology. 42(3):1076–1084.
Gaol, Y. E. L., Erly., dan Elmatris, S.Y. 2014. Pola Resistensi Bakteri Aerob Pada Ulkus Diabetik Terhadap Beberapa Antibiotika di Laboratorium Mikrobiologi RSUP Dr. M. Djamil Padang Tahun 2011-2013, Jurnal Kesehatan Andalas, 6(1).
Gilman, Goodman A. 2012, Goodman & Gilman. Dasar Farmakologi Terapi, Ed 10, Jakarta :EGC
Harley, P. 2002 Laboratory Exercises in Microbiology, 5th edition, 257-260. New York: McGraw-Hill Companies.
Hastuti, R., T. 2008. Faktor-Faktor Risiko Ulkus Diabetika pada Penderita Diabetes Mellitus (Studi Kasus di RSUD Dr. Moewardi Surakarta). [Tesis]. Universitas Diponegoro, Semarang.
International Diabetes Federation (IDF). 2011. IDF Diabetes Atlas, 5th Edition, International Diabetes Federation.
Jawetz., Melnick., dan Adelberg. 2010. Mikrobiologi Kedokteran. Jakarta: EGC.
Kemenkes RI. 2013. Riset Kesehatan Dasar. Badan Penelitian & Pengembangan Kementrian Kesehatan RI, Jakarta.
Kontopoulou, K., Protonotariou, E., Vasilakos, K., Kriti, M., Koteli, A., Antoniadou, E., Safianou, D. 2010. Hospital Outbreak Caused By Klebsiella pneumonia Producing KPC-2 Beta-lactamase Resistant To Colistin. J Hosp Infect, 76(1) : 70-73
Lipsky, B., A., Berendt A., R., Cornia P., B., Pile, J., C., Peters, E., J., G., dan Armstrong, D., G. 2012. Infection Diseases Society of America Clinical Practice Guideline for the Diagnosis and Treatment of Diabetic Foot Infections, Clinical Infectious Diseases. 54 (12) : 132-173.
Ludden, C., Cormican, M., Vellinga, A., Johnson, J. R., Austin, B., dan Morris, D. 2015. Colonisation With ESBL – Producing And Carbapenemase –Producing Enterobacteriaceae, Vancomycin – Resistant Enterococci, And Meticillin – Resistant Staphylococcus Aureus In A Long-Tern Care Facility Over One Year. BMC Infect Dis. 15(168).
McPhee, S. J., dan Ganong, W. F. 2010. Patofisiologi Penyakit Pengantar Menuju Kedokteran Klinis, Edisi 5. Jakarta: EGC.
Nur, A., dan Marissa, N. 2016. Gambaran Bakteri Ulkus Diabetikum di Rumah Sakit Zainal Abidin dan Merauxa Tahun 2015, Buletin Penelitian Kesehatan. 44(3) : 187-196.
Patil, Y., Khadse, R., Chavan, S., dan Raut,S. 2018, Bacteriological Profil of Diabetic Foot Infections, European Journal of Pharmaceutical and Medical Research. 5 (6) : 631-635.
Riset Kesehatan Dasar (Riskesdas). 2013. Pedoman Pewancara Petugas Pengumpul Data, Badan Litbangkes Depkes RI, Jakarta.
Rizal. 2010. Microbial Pattern And Antimicrobial Resistance Of Isolates Collected From Various Specimen In DR. Oen Solo Baru Hospital, Sukoharjo, The Indonesian Journal Of Medical Science. 1(7) : 392.
Setianingsih, Y., Fadraersada, J., Ibrahim, A., dan Ramadhan, A. M. 2016. Pola Resistensi Bakteri Terhadap Antibiotik Pada Pasien Diabetic Foot Di RSUP Abdul Wahab Sjahranie Samarinda Periode Agustus-Oktober 2016, Prosiding Seminar Nasional Kefarmasian ke-4: 407-414.
Setiawan, D. S. 2010. Faktor Resiko Koloni sasi Enterobacteriaceae Pada Nasofaring Manusia Dewasa, Universitas Diponegoro, Semarang.
Suhartati, R., dan Faidah, E. N. 2014. Identifikasi Bakteri Oxacillin Resistant Staphylococcus Aureus (Orsa) Pada Ulkus Penderita Diabetes Mellitus Di Ruang Perawatan Bedah Rumah Sakit Umum Daerah (RSUD) Tasikmalaya, Jurnal Kesehatan Bakti Tunas Husada. 11 (1) : 161-167.
Waspadji, S. 2012. Komplikasi Kronik Diabetes: Mekanisme Terjadinya, Diagnosis & Strategi Pengelolaan. Jakarta: EGC.
Yunir, Soebardi. 2006. Buku Ajar Ilmu Penyakit Dalam Jilid III Ed. 4. Jakarta: Balai penerbit FKU.
Downloads
Published
31-12-2019
How to Cite
Novelni, R. (2019). IDENTIFIKASI DAN UJI RESISTENSI BAKTERI PADA PASIEN ULKUS DIABETIKUM DI BANGSAL INTERNE RSUP DR. M. DJAMIL PADANG. Jurnal Penelitian Farmasi Indonesia, 8(2), 67–74. https://doi.org/10.51887/jpfi.v8i2.550
Issue
Section
Articles
