PENGARUH PEMBERIAN INFUSA BATANG GELAGAH (Saccharum spontaneum L.) TERHADAP KADAR GLUKOSA DARAH MENCIT PUTIH (Mus musculus L.) JANTAN YANG DIINDUKSI GLUKOSA
DOI:
https://doi.org/10.51887/jpfi.v10i1.976Keywords:
reeds infusion, metformin, glucose, Oral Glucose Tolerance Test (TTGO)Abstract
A study on the effect of reeds infusion (Saccharum spontaneum L.) on glucose levels of male white mice (Mus musculus) given glucose load. This study aims to see the effect of reeds infusion (Saccharum spontaneum L.) to blood glucose levels of male white mice (Mus musculus) given glucose load as much as 2g/KgBB. This research used Oral Glucose Tolerance Test (TTGO) method. Animal experiments were divided into 6 groups. The negative control group was given only aquadest, the comparison group was given 2g/KgBB glucose solution, the positive group was given metformin with the dosage of 65mg/KgBB, the treatment group was given reeds infusion (Saccharum spontaneum L.) with concentration 10, 20 and 30 %. After each test animal was given 30 minutes treatment then given glucose 2g/KgBB orally. Then measured blood glucose levels of mice at minute 30-180. Based on the results of testing the infusion of reeds (Saccharum spontaneum L..) showed that the concentrations of 10, 20 and 30% had decreased levels of white mice blood glucose (Mus musculus L.). However, the results of the two way ANOVA assay continued with the Post Hoc Tukey test showed the results obtained that the duration of administration may affect the decrease in blood glucose levels of mice (p <0.05).
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References
Depkes RI 1995. Farmakope Indonesia. Edisi ke-IV. Jakarta: Departemen Kesehatan Republik Indonesia.
Depkes RI 2005. Pharmaceutical care untuk Penyakit Diabetes Melitus. Jakarta: Departemen Kesehatan Republik Indonesia.
Devi, J.A.I. & Muthu, K. 2014. Phytochemical Screening, Antioxidant Activities and Total Phenolic Content of Ethanolic Extract from Whole Plant of Saccharum spontaneum (Linn.). International Journal of Chemical and Pharmaceutical Sciences, 5(2): 112–118.
Dheer, R. & Bhatnagar, P. 2010. A Study of The Antidiabetic Activity of Barleria Prionitis Linn. Indian Journal of Pharmacology, 42(2): 70–73.
Dipiro, J.T., Talbert, R.L., Yee, G.C., Matzke, G.R., Wells, B.G. & Posey, L.M. 2015. Pharmacotherapy:A Pathophysiologic Approach, 10th edition. New York: Mc Graw Hill.
Harborne, J.. 1987. Metode Kimia Cara Modern Menganalisis Tumbuhan. Bandung: ITB.
Hidayatullah, M. & Mora, E. 2018. Uji Aktivitas Antioksidan Ekstrak Etanol Batang Gelagah (Saccharum spontaneum L) dengan Metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Jurnal Penelitian Farmasi Indonesia, 1(1): 1–5.
Kemenkes RI 2018. Riset Kesehatan Dasar (RISKESDAS). Badan Penelitian dan Pengembangan Kesehatan Kementerian RI Tahun 2018.
Lapuz, A., Arabiran, R., Sembrano, T., Albaniel, J., Paet, J. & Maini, H. 2016. Preformulation and Evaluation of Antibacterial and Anti-Inflammatory Activities of Saccharum spontaneum Linne Root Extract Cream. International Journal of Chemical Engineering and Applications, 7(3): 204–208.
Liang, Y., Osborne, M.C., Monia, B.P., Bhanot, S., Gaarde, W.A., Reed, C., She, P., Jetton, T.L. & Demarest, K.T. 2004. Reduction in Glucagon Receptor Expression by an Antisense Oligonucleotide Ameliorates Diabetic Syndrome in db/db Mice. Image (Rochester, N.Y.), 53: 367–374.
Norcahyati 2012. Tumbuhan Berkhasiat Obat Etnis Asli Kalimantan. Samboja: Kementrian Kehutanan.
Nugroho, A.E. 2006. Animal Models of Diabetes Mellitus : Pathology and Mechanism of Some Diabetogenics. Biodiversitas, Journal of Biological Diversity, 7(4): 378–382.
Perkeni 2015. Konsensus Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Di Indonesia 2015. Jakarta: Pengurus Besar Perkumpulan Endokrinologi Indonesia.
Rahayuningsih, N. & Amelia, S. 2015. Uji Aktivitas Antidiabetes Infusa Daun Pohpohan (Pilea trinervia Wight.) Pada Mencit Putih Jantan Galur Swiss webster. Jurnal Kesehatan Bakti Tunas Husada, 13(1).
Ridwan, E. 2013. Etika Pemanfaatan Hewan Percobaan Dalam Penelitian Kesehatan. J indon Med Assoc, 63(3).
Ripa, F.A., Haque, M. & Haque, M.I.U. 2009. In vitro Antimicrobial, Cytotoxic and Antioxidant Activity of Flower Extract of Saccharum spontaneum Linn. European Journal of Scientific Research, 30(3): 478–483.
Song, J., Kwon, O., Chen, S., Daruwala, R., Eck, P., Park, J.B. & Levine, M. 2002. Flavonoid inhibition of SVCT1 and GLUT2, Intestinal Trasporters for Vitamin C and Glucose. J.Biol, 1: 303–315.
Tag, H., Kalita, P., Dwivedi, P., Das, A.K. & Namsa, N.D. 2012. Herbal medicines used in the treatment of diabetes mellitus in Arunachal Himalaya , northeast , India. Journal of Ethnopharmacology, 141(3): 786–795.
Wijayakusuma, H. 1995. Tanaman Berkhasiat Obat di Indonesia Jilid II. Jakarta: Pustaka Kartini.
