SINTESIS DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA 3ꞌ-BROMO-4-METOKSI KALKON
DOI:
https://doi.org/10.51887/jpfi.v11i2.1547Abstract
Chalcone is a flavonoid derivative that has a variety of interesting biological activities. One of the compounds suspected of being an antibacterial agent is chalcone. The properties of chalcone compounds depend on the substituents that depend on the two aromatic rings, such as Cl, Br, OH groups and so on. The purpose of this study was to synthesize chalcone analogue compounds and determine the potential of these analog compounds as antibiotics. In this research, a chalcone analogue compound has been successfully synthesized, namely 3ꞌ-Bromo-4-methoxy-chalcone through the stirring method using KOH catalyst, ethanol solvent. The results of the synthesis carried out by recrystallization and the yield obtained were 64 %. Identification of compounds that have been carried out by TLC test, melting point, and HPLC analysis. The structure of the compound was characterized by UV-Vis spectroscopy, FTIR and 1H NMR, indicating that the analogue chalcone compound has a structure as expected. Testing the activity of chalcone analogue compounds was carried out on Staphylococcus aureus and Escherichia coli at a concentration of 1%, 5%, 10% and 20% test solutions. The test results showed that the antibacterial activity of the compound 3ꞌ-Bromo-4-methoxy-chalcone was categorized as weak.
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References
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Nather, S.. 2012. Evaluation of Antibacterial Activity of Morinda citrifolia, Vilextrifolia, and Chromolaenaadorata. African Journal of Pharmacy and Pharmacology, 6(11): 783–788.
Poeloengan, M. & Pratiwi 2010. Uji Aktivitas Antibakteri Ekstrak Kulit Buah Mangga (Garcinia mangostana Linn). Media Litbang Kesehatan, 20(2): 65–69.
Pretsch, erno., Philippe Buhlmann., M.B. 2020. Structure Determination of Organic Compounds. Fifth Edit ed. Germany: Springer Spektrum.
Roch, M., Clair, P., Renzoni, A., Reverdy, M., Dauwalder, O. & Bes, M. 2014. Exposure of Staphylococcus aureus to Subinhibitory Concentrations of  -Lactam Antibiotics Induces Heterogeneous Vancomycin- Intermediate Staphylococcus aureus. 58(9): 5306–5314.
Tiwari, V., Singh, J.P., Sharma, P., Dangi, L. & Dulawat, S.S. 2011. Microwave Assisted Improved Synthesis of Chalcones Under Microwave Irradiation and Their Antibacterial Activity. Journal of Chemical, Biological and Physical Sciences, 1(1): 22–27.
Balkrishna, Pratapwar, A., Tapas, A., Butle, S. & Vatkar, B. 2014. Synthesis and Antimicrobial Activity of Novel Chalcone Derivatives. Research on Chemical Intermediates, 40(4): 1715–1725.
Brahmana, E.M. 2015. Sintesis dan Uji Antibakteri Senyawa (E)-1-(2-Klorofenil)-3-Ptolilprop-2-en-1-on. Jurnal Ilmiah Edu Research, 4(2): 103–108.
Dona, R., Zamri, A. & Jasril 2015. Sintesis dan Uji Toksisitas Senyawa Analog Kalkon Tersubstitusi Metoksi. Jurnal Photon, 5(2): 9–14.
Fikroh, R.A., Matsjeh, S. & Anwar, C. 2020. Aktivitas Antibakteri Turunan Kalkon Tersubstitusi Bromo Terhadap Bakteri Bacillus subtilis dan
Jasril, J., Teruna, H.Y., Zamri, A., Alfatos, D., Yuslinda, E. & Nurulita, Y. 2013. Sintesis dan Uji Antibakteri Senyawa Bromo Kalkon Piridin. Jurnal Natur Indonesia, 14(3): 172–175.
Nather, S.. 2012. Evaluation of Antibacterial Activity of Morinda citrifolia, Vilextrifolia, and Chromolaenaadorata. African Journal of Pharmacy and Pharmacology, 6(11): 783–788.
Poeloengan, M. & Pratiwi 2010. Uji Aktivitas Antibakteri Ekstrak Kulit Buah Mangga (Garcinia mangostana Linn). Media Litbang Kesehatan, 20(2): 65–69.
Pretsch, erno., Philippe Buhlmann., M.B. 2020. Structure Determination of Organic Compounds. Fifth Edit ed. Germany: Springer Spektrum.
Roch, M., Clair, P., Renzoni, A., Reverdy, M., Dauwalder, O. & Bes, M. 2014. Exposure of Staphylococcus aureus to Subinhibitory Concentrations of  -Lactam Antibiotics Induces Heterogeneous Vancomycin- Intermediate Staphylococcus aureus. 58(9): 5306–5314.
Tiwari, V., Singh, J.P., Sharma, P., Dangi, L. & Dulawat, S.S. 2011. Microwave Assisted Improved Synthesis of Chalcones Under Microwave Irradiation and Their Antibacterial Activity. Journal of Chemical, Biological and Physical Sciences, 1(1): 22–27.
